期刊
TOXINS
卷 10, 期 8, 页码 -出版社
MDPI
DOI: 10.3390/toxins10080327
关键词
aflatoxins; maize; households; markets; immunosensor; HPLC; ELISA
资金
- Uganda Industrial Research Institute
- VU University Amsterdam
- TNO
- Yoba for Life foundation
In sub-Saharan Africa, there is a high demand for affordable and accessible methods for on-site detection of aflatoxins for appropriate food safety management. In this study, we validated an electrochemical immunosensor device by the on-site detection of 60 maize flour samples from six markets and 72 samples from households in Kampala. The immunosensor was successfully validated with a linear range from 0.7 +/- 0.1 to 11 +/- 0.3 mu g/kg and limit of detection (LOD) of 0.7 mu g/kg. The maize flour samples from the markets had a mean total aflatoxin concentration of 7.6 +/- 2.3 mu g/kg with approximately 20% of the samples higher than 10 mu g/kg, which is the maximum acceptable level in East Africa. Further down the distribution chain, at the household level, approximately 45% of the total number contained total aflatoxin levels higher than the acceptable limit. The on-site detection method correlated well with the established laboratory-based HPLC and ELISA-detection methods for aflatoxin B-1 with the correlation coefficients of 0.94 and 0.98, respectively. This study shows the feasibility of a novel on-site detection method and articulates the severity of aflatoxin contamination in Uganda.
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