期刊
TOXINS
卷 5, 期 11, 页码 2093-2108出版社
MDPI
DOI: 10.3390/toxins5112093
关键词
okadaic acid; histopathology; diarrhea; immunohistochemistry; liver; superoxide dismutase 1; catalase; quantitative PCR; mice
资金
- Ministerio de Ciencia y Tecnologia, Spain [AGL2009-13581-CO2-01, AGL2012-40485-CO2-01]
- Xunta de Galicia, Spain [10PXIB261254 PR]
- European Union's Seventh Framework Programme [265896 BAMMBO, 265409 muAQUA, 262649 BEADS, 315285 Ciguatools, 312184 PharmaSea]
- Atlantic Area Programme (Interreg IVB Trans-national) [2009-1/117 Pharmatlantic]
In vivo, after administration by gavage to mice and rats, okadaic acid has been reported to produce lesions in liver, small intestine and forestomach. Because several reports differ in the damage detected in different organs, and on okadaic acid distribution after consumption, we determined the toxicity of this compound after oral administration to mice. After 24 hours, histopathological examination showed necrotic foci and lipid vacuoles in the livers of intoxicated animals. By immunohistochemical analysis, we detected this toxin in the liver and kidneys of intoxicated animals. Okadaic acid induces oxidative stress and can be activated in vitro into reactive compounds by the post-mitochondrial S9 fraction, so we studied the okadaic effect on the gene expression of antioxidant and phase II detoxifying enzymes in liver. We observed a downregulation in the expression of these enzymes and a reduction of protein expression of catalase and superoxide dismutase 1 in intoxicated animals.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据