Ochratoxin A in Roasted Coffee from French Supermarkets and Transfer in Coffee Beverages: Comparison of Analysis Methods

The OTA content of 30 roasted coffees purchased in French supermarkets was evaluated by two validated different methods: one using immunoaffinity column (IAC) clean-up after alkaline extraction; the second using toluene extraction under acidic conditions. OTA recoveries (0.5 to 5 mu g/kg) ranged from 16-49% with the alkaline extraction method and 55-60% with the acidic method. OTA recoveries from prepared beverages were similar with all methods (75-80%). All samples containing OTA ranged from trace (