期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 2, 期 4, 页码 246-254出版社
WILEY
DOI: 10.5966/sctm.2012-0081
关键词
Embryonic stem cells; Cell culture; Xenograft assay; Cell survival; Induced pluripotent stem cells
资金
- Ministry of Education, Youth, and Sport of the Czech Republic [MSMT0021622430, LC06077]
- Grant Agency of the Czech Republic [302/12/G157]
- European Regional Development Fund [FNUSA-ICRC CZ.1.05/1.1.00/02.0123]
- SoMoPro
- European Community [229603]
- South Moravian Region
The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as a single-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system is highly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies. STEM CELLS TRANSLATIONAL MEDICINE 2013;2:246-254
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