期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 1, 期 3, 页码 248-260出版社
WILEY
DOI: 10.5966/sctm.2011-0028
关键词
Cardiac; Tissue regeneration; Stem cell transplantation; Embryonic stem cells
资金
- Leenaards Foundation
- Swiss National Science Foundation FNS [4046-058712, PP00B-68778/1]
- Swiss National Science Foundation [310030_141162, CR32I3_129987]
- European Union [BETAIMAGE 222980, IMIDIA, C2008-T7]
- Swiss National Science Foundation (SNF) [310030_141162] Funding Source: Swiss National Science Foundation (SNF)
Pluripotent stem cell-seeded cardiopatches hold promise for in situ regeneration of infarcted hearts. Here, we describe a novel cardiopatch based on bone morphogenetic protein 2-primed cardiac-committed mouse embryonic stem cells, embedded into biodegradable fibrin matrices and engrafted onto infarcted rat hearts. For in vivo tracking of the engrafted cardiac-committed cells, superparamagnetic iron oxide nanoparticles were magnetofected into the cells, thus enabling detection and functional evaluation by high-resolution magnetic resonance imaging. Six weeks after transplantation into infarcted rat hearts, both local (p < .04) and global (p < .015) heart function, as well as the left ventricular dilation (p < .0011), were significantly improved (p < .001) as compared with hearts receiving cardiopatches loaded with iron nanoparticles alone. Histological analysis revealed that the fibrin scaffolds had degraded over time and clusters of myocyte enhancer factor 2-positive cardiac-committed cells had colonized most of the infarcted myocardium, including the fibrotic area. De novo CD31-positive blood vessels were formed in the vicinity of the transplanted cardiopatch. Altogether, our data provide evidence that stem cell-based cardiopatches represent a promising therapeutic strategy to achieve efficient cell implantation and improved global and regional cardiac function after myocardial infarction. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:248-260
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