期刊
STEM CELLS TRANSLATIONAL MEDICINE
卷 1, 期 10, 页码 740-749出版社
WILEY
DOI: 10.5966/sctm.2012-0061
关键词
Cell biology; Multipotential differentiation; Monocyte; Mesenchymal stem cells
资金
- University of Wisconsin-Madison School of Pharmacy
- NIH [EB6613]
Bone marrow mesenchymal stromal/stem cell (MSC) encapsulation within a biomatrix could improve cellular delivery and extend survival and residence time over conventional intravenous administration. Although MSCs modulate monocyte/macrophage (Mempty set) immunophenotypic properties, little is known about how such interactions are influenced when MSCs are entrapped within a biomaterial. Furthermore, the impact of the cell-encapsulating matrix on MSC multipotency and on Mempty sets, which infiltrate biomaterials, remains poorly understood. Here we elucidate this three-way interaction. The Mempty set immunophenotype and MSC differentiation were examined with regard to established and experimental collagen-based biomaterials for MSC entrapment. Tumor necrosis factor-alpha secretion was acutely inhibited at 4 days. MSCs cocultured with Mempty sets demonstrated attenuated chondrocyte differentiation, whereas osteoblast differentiation was enhanced. Adipocyte differentiation was considerably enhanced for MSCs entrapped within the gelatin/polyethylene glycol-based matrix. A better understanding of the effect of cell encapsulation on differentiation potency and immunomodulation of MSCs is essential for MSC-based, biomaterial-enabled therapies. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:740-749
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