期刊
STEM CELL REPORTS
卷 4, 期 3, 页码 503-518出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2015.01.015
关键词
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资金
- BioRN Spitzencluster Molecular and Cell Based Medicine
- German Bundesministerium fur Bildung und Forschung (BMBF)
- Deutsche Forschungsgemeinschaft (DFG) [SFB 873]
- Dietmar-Hopp Foundation
Methods to isolate and culture primary prostate epithelial stem/progenitor cells (PESCs) have proven difficult and ineffective. Here, we present a method to grow and expand both murine and human basal PESCs long term in serum- and feeder-free conditions. The method enriches for adherent mouse basal PESCs with a Lin(-)SCA-1(+)CD49f(+)TROP2(high) phenotype. Progesterone and sodium selenite are additionally required for the growth of human Lin(-)CD49f(+)TROP2(high) PESCs. The gene-expression profiles of expanded basal PESCs show similarities to ESCs, and NF-kB function is critical for epithelial differentiation of sphere-cultured PESCs. When transplanted in combination with urogenital sinus mesenchyme, expanded mouse and human PESCs generate ectopic prostatic tubules, demonstrating their stem cell activity in vivo. This novel method will facilitate the molecular, genomic, and functional characterization of normal and pathologic prostate glands of mouse and human origin.
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