期刊
REVISTA BRASILEIRA DE PARASITOLOGIA VETERINARIA
卷 22, 期 2, 页码 289-291出版社
BRAZILIAN COLL VETERINARY PARASITOLOGY
DOI: 10.1590/S1984-29612013000200049
关键词
Cytauxzoonosis; hemoplasmosis; PCR; sequencing; feline
资金
- FAPERJ
- CAPES
- CNPq
This article describes the first detection of Cytauxzoon felis, using Molecular techniques, in a naturally infected domestic cat from Brazil, South America. Coinfection with Candidatus Mycoplasma haemominutum' was also found. The molecular identification of the piroplasmid species was performed by Polymerase Chain Reaction (PCR) and sequencing analysis. A 284 pb fragment of the gene encoding the 18S ribosomal RNA region was amplified and showed 99% identity with other C. felis strains from North America. In addition, PCR-RFLP (restriction fragment length polymorphism) analysis, which amplifies a 595 bp fragment of the gene encoding 16S ribosomal RNA of some bacterial species, identified the co-infecting species as Candidatus M. haemominutum'.
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