期刊
PROTEIN & CELL
卷 3, 期 1, 页码 71-79出版社
HIGHER EDUCATION PRESS
DOI: 10.1007/s13238-012-2007-8
关键词
human embryonic stem cells (hESCs); mouse ESCs; naive; pluripotent state
类别
资金
- China National Basic Research Program [2011CB965300]
- National Natural Science Foundation of China [90919060]
- Strategic Priority Research Program of the Chinese Academy of Sciences [XDA01020101]
The pluripotent state between human and mouse embryonic stem cells is different. Pluripotent state of human embryonic stem cells (ESCs) is believed to be primed and is similar with that of mouse epiblast stem cells (EpiSCs), which is different from the naive state of mouse ESCs. Human ESCs could be converted into a naive state through exogenous expression of defined transcription factors (Hanna et al., 2010). Here we report a rapid conversion of human ESCs to mouse ESC-like naive states only by modifying the culture conditions. These converted human ESCs, which we called mhESCs (mouse ESC-like human ESCs), have normal karyotype, allow single cell passage, exhibit domed morphology like mouse ESCs and express some pluripotent markers similar with mouse ESCs. Thus the rapid conversion established a naive pluripotency in human ESCs like mouse ESCs, and provided a new model to study the regulation of pluripotency.
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