期刊
PROTEIN & CELL
卷 2, 期 5, 页码 410-422出版社
HIGHER EDUCATION PRESS
DOI: 10.1007/s13238-011-1049-7
关键词
Rad1; DNA damage; checkpoint signaling; DNA repair; homologous recombination repair
类别
资金
- National Natural Science Foundation of China [30900813]
- Knowledge Innovation Program of Chinese Academy of Sciences [KSCX2-YW-R63]
The Rad1 gene is evolutionarily conserved from yeast to human. The fission yeast Schizosaccharomyces pombe Rad1 ortholog promotes cell survival against DNA damage and is required for G(2)/M checkpoint activation. In this study, mouse embryonic stem (ES) cells with a targeted deletion of Mrad1, the mouse ortholog of this gene, were created to evaluate its function in mammalian cells. Mrad1(-/-) ES cells were highly sensitive to ultraviolet-light (UV light), hydroxyurea (HU) and gamma rays, and were defective in G(2)/M as well as S/M checkpoints. These data indicate that Mrad1 is required for repairing DNA lesions induced by UV-light, HU and gamma rays, and for mediating G(2)/M and S/M checkpoint controls. We further demonstrated that Mrad1 plays an important role in homologous recombination repair (HRR) in ES cells, but a minor HRR role in differentiated mouse cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据