期刊
PROTEIN & CELL
卷 1, 期 2, 页码 198-204出版社
SPRINGEROPEN
DOI: 10.1007/s13238-010-0028-8
关键词
nsp8; SARS-CoV; RNA primase; viral life cycle
类别
资金
- Project 973 of the Ministry of Science and Technology of China [2006CB806503, 2007CB914301]
- National Natural Science Foundation of China [30221003, 30730022]
During severe acute respiratory syndrome coronavirus (SARS-CoV) infection, the activity of the replication/transcription complexes (RTC) quickly peaks at 6 hours post infection (h.p.i) and then diminishes significantly in the late post-infection stages. This down-up-down regulation of RNA synthesis distinguishes different viral stages: primary translation, genome replication, and finally viron assembly. Regarding the nsp8 as the primase in RNA synthesis, we confirmed that the proteolysis product of the primase (nsp8) contains the globular domain (nsp8C), and indentified the resectioning site that is notably conserved in all the three groups of coronavirus. We subsequently crystallized the complex of SARS-CoV nsp8C and nsp7, and the 3-D structure of this domain revealed its capability to interfuse into the hexadecamer super-complex. This specific proteolysis may indicate one possible mechanism by which coronaviruses to switch from viral infection to genome replication and viral assembly stages.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据