期刊
POLYMERS
卷 6, 期 10, 页码 2510-2525出版社
MDPI AG
DOI: 10.3390/polym6102510
关键词
cryogels; alpha-tricalcium phosphate; PDMAEMA (Poly(dimethyl aminoethyl methacrylate)); PHEMA (poly(hydroxyethyl methacrylate); mesenchymal stem cells
资金
- Comision Interministerial de Ciencia y Tecnologia (CICYT), Spain [MAT2010-18155]
- PLE [2009-0144]
- ACI-PROMOCIONA [ACI2010-1095]
- Ministry of Science and Education (MICINN), Spain
- Brazilian National Council for Scientific and Technological Development (CNPq) [202018/2008-3]
The aim of this work is to evaluate the potential of cryogels to be used as scaffolds in tissue engineering. Scaffolds based on the a-tricalcium phosphate reinforced PDMAEMA (Poly(dimethyl aminoethyl methacrylate))/PHEMA (poly(hydroxyethyl methacrylate)) system were prepared and human trabecular bone-derived cells (HTBs) and bone marrow derived-mesenchymal stem cells (BM-MSCs) cultured on them. Several features, such as porosity, pore shape, molecular weight between crosslinks and mesh size, are studied. The most suitable PDMAEMA/PHEMA ratio for cell proliferation has been assessed and the viability, adhesion, proliferation and expression of osteoblastic biochemical markers are evaluated. The PDMAEMA/PHEMA ratio influences the scaffolds porosity. Values between 53% +/- 5.7% for a greater content in PHEMA and 75% +/- 5.5% for a greater content in PDMAEMA have been obtained. The polymer ratio also modifies the pore shape. A greater content in PDMAEMA leads also to bigger network mesh size. Each of the compositions were non-cytotoxic, the seeded cells remained viable for both BM-MSCs and HTBs. Thus, and based on the structural analysis, specimens with a greater content in PDMAEMA seem to provide a better structural environment for their use as scaffolds for tissue engineering. The a-tricalcium phosphate incorporation into the composition seems to favor the expression of the osteogenic phenotype.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据