期刊
PEERJ
卷 3, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.740
关键词
KSB3 phylum; Candidate phylum; Wastewater treatment; Anaerobic biotechnology; Filamentous bulking; Metagenomics
资金
- Biomedical Research Institute of the National Institute of Advanced Industrial Science and Technology (AIST)
- Australian Research Council (ARC) Discovery Outstanding Researcher Award (DORA) [DP120103498]
- ARC Queen Elizabeth II Fellowship [DP1093175]
- Natural Sciences and Engineering Research Council of Canada
Filamentous cells belonging to the candidate bacterial phylum KSB3 were previously identified as the causative agent of fatal filament overgrowth (bulking) in a high-rate industrial anaerobic wastewater treatment bioreactor. Here, we obtained near complete genomes from two KSB3 populations in the bioreactor, including the dominant bulking filament, using differential coverage binning of metagenomic data. Fluorescence in situ hybridization with 16S rRNA-targeted probes specific for the two populations confirmed that both are filamentous organisms. Genome-based metabolic reconstruction and microscopic observation of the KSB3 filaments in the presence of sugar gradients indicate that both filament types are Gram-negative, strictly anaerobic fermenters capable of non-flagellar based gliding motility, and have a strikingly large number of sensory and response regulator genes. We propose that the KSB3 filaments are highly sensitive to their surroundings and that cellular processes, including those causing bulking, are controlled by external stimuli. The obtained genomes lay the foundation for a more detailed understanding of environmental cues used by KSB3 filaments, which may lead to more robust treatment options to prevent bulking.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据