期刊
PEERJ
卷 3, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.1498
关键词
Insulin secretion; Volatile anesthetic; Panreactic beta-cell; HIF-1; MIN6 cell; ATP
资金
- JSPS [24659695, 22659283, 25462457, 24592322, 15K10551]
- Grants-in-Aid for Scientific Research [15K20063, 25462457, 15K10551, 22659283, 24592322, 24659695, 26670693] Funding Source: KAKEN
Proper glycemic control is one of the most important goals in perioperative patient management. Insulin secretion from pancreatic beta-cells in response to an increased blood glucose concentration plays the most critical role in glycemic control. Several animal and human studies have indicated that volatile anesthetics impair glucose-stimulated insulin secretion (GSIS). A convincing GSIS model has been established, in which the activity of ATP-dependent potassium channels (K-ATP) under the control of intracellular ATP plays a critical role. We previously reported that pimonidazole adduct formation and stabilization of hypoxia-inducible factor-1 alpha (HIF-1 alpha) were detected in response to glucose stimulation and that MIN6 cells overexpressing HIF-1 alpha were resistant to glucose-induced hypoxia. Genetic ablation of HIF-1 alpha or HIF-1 beta significantly inhibited GSIS in mice. Moreover, we previously reported that volatile anesthetics suppressed hypoxia-induced HIF activation in vitro and in vivo. To examine the direct effect of volatile anesthetics on GSIS, we used the MIN6 cell line, derived from mouse pancreatic beta-cells. We performed a series of experiments to examine the effects of volatile anesthetics (sevoflurane and isoflurane) on GSIS and demonstrated that these compounds inhibited the glucose-induced ATP increase, which is dependent on intracellular hypoxia-induced HIF-1 activity, and suppressed GSIS at a clinically relevant dose in these cells.
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