期刊
PLOS PATHOGENS
卷 10, 期 5, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1004131
关键词
-
资金
- DFG [He 2526/6-2]
- European Commission [QLRT-2001-01112, MRTN-CT-2005-019248]
- Helmholtz Association [VISTRIE VH-VI-242]
- German Academic Exchange Service (DAAD)
- Dusseldorf Entrepreneur Foundation
Human cytomegalovirus (HCMV) establishes lifelong infection with recurrent episodes of virus production and shedding despite the presence of adaptive immunological memory responses including HCMV immune immunoglobulin G (IgG). Very little is known how HCMV evades from humoral and cellular IgG-dependent immune responses, the latter being executed by cells expressing surface receptors for the Fc domain of IgG (Fc gamma Rs). Remarkably, HCMV expresses the RL11-encoded gp34 and UL119-118-encoded gp68 type I transmembrane glycoproteins which bind Fc gamma with nanomolar affinity. Using a newly developed Fc gamma R activation assay, we tested if the HCMV-encoded Fc gamma binding proteins (HCMV Fc gamma Rs) interfere with individual host Fc gamma Rs. In absence of gp34 or/and gp68, HCMV elicited a much stronger activation of Fc gamma RIIIA/CD16, Fc gamma RIIA/CD32A and Fc gamma RI/CD64 by polyclonal HCMV-immune IgG as compared to wildtype HCMV. gp34 and gp68 co-expression culminates in the late phase of HCMV replication coinciding with the emergence of surface HCMV antigens triggering Fc gamma RIII/CD16 responses by polyclonal HCMV-immune IgG. The gp34- and gp68-dependent inhibition of HCMV immune IgG was fully reproduced when testing the activation of primary human NK cells. Their broad antagonistic function towards Fc gamma RIIIA, Fc gamma RIIA and Fc gamma RI activation was also recapitulated in a gain-of-function approach based on humanized monoclonal antibodies (trastuzumab, rituximab) and isotypes of different IgG subclasses. Surface immune-precipitation showed that both HCMV-encoded Fc gamma binding proteins have the capacity to bind trastuzumab antibody-HER2 antigen complexes demonstrating simultaneous linkage of immune IgG with antigen and the HCMV inhibitors on the plasma membrane. Our studies reveal a novel strategy by which viral Fc gamma Rs can compete for immune complexes against various Fc receptors on immune cells, dampening their activation and antiviral immunity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据