期刊
PLOS PATHOGENS
卷 8, 期 8, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.ppat.1002872
关键词
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资金
- VIDI fellowship [864.08.003]
- Open Program of the Division for Earth and Life Sciences, Netherlands Organization for Scientific Research [821.02.028]
- Nijmegen Centre for Molecular Life Sciences of the Radboud University Nijmegen Medical Centre
- Centre National de la Recherche Scientifiques
- Agence Nationale de la Recherche (ANR - Nuclear-endosiRNAs)
- Swedish Research Council
- EU
- Swedish Cancer Society
- Academy of Finland
- Sigrid Juselius Foundation
- Netherlands Organization for Scientific Research
RNA interference (RNAi) is a major antiviral pathway that shapes evolution of RNA viruses. We show here that Nora virus, a natural Drosophila pathogen, is both a target and suppressor of RNAi. We detected viral small RNAs with a signature of Dicer-2 dependent small interfering RNAs in Nora virus infected Drosophila. Furthermore, we demonstrate that the Nora virus VP1 protein contains RNAi suppressive activity in vitro and in vivo that enhances pathogenicity of recombinant Sindbis virus in an RNAi dependent manner. Nora virus VP1 and the viral suppressor of RNAi of Cricket paralysis virus (1A) antagonized Argonaute-2 (AGO2) Slicer activity of RNA induced silencing complexes pre-loaded with a methylated single-stranded guide strand. The convergent evolution of AGO2 suppression in two unrelated insect RNA viruses highlights the importance of AGO2 in antiviral defense.
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