期刊
PLOS GENETICS
卷 8, 期 4, 页码 587-600出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1002678
关键词
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资金
- National Science Foundation [0445684]
- Jeffress Memorial Trust [J-840]
- National Institutes of Health [R15 5GM096309]
- Howard Hughes Medical Institute (HHMI)
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0445684] Funding Source: National Science Foundation
The sperm/oocyte decision in the hermaphrodite germline of Caenorhabditis elegans provides a powerful model for the characterization of stem cell fate specification and differentiation. The germline sex determination program that governs gamete fate has been well studied, but direct mediators of cell-type-specific transcription are largely unknown. We report the identification of spe-44 as a critical regulator of sperm gene expression. Deletion of spe-44 causes sperm-specific defects cytokinesis, cell cycle progression, and organelle assembly resulting in sterility. Expression of spe-44 correlates precisely with spermatogenesis and is regulated by the germline sex determination pathway. spe-44 is required for the appropriate expression of several hundred sperm-enriched genes. The SPE-44 protein is restricted to the sperm-producing germline where it localizes to the autosomes (which contain sperm genes) but is excluded from the transcriptionally silent. X chromosome (which does not). The orthologous gene in other Caenorhabditis species is similarly expressed in a sex-biased manner, and the protein likewise exhibits autosome-specific localization in developing sperm, strongly suggestive of an evolutionarily conserved role in sperm gene expression. Our analysis represents the first identification of a transcriptional regulator whose primary function is the control of gamete-type-specific transcription in this system.
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