期刊
PLOS GENETICS
卷 6, 期 1, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pgen.1000793
关键词
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资金
- Ministere de la Recherche et de l'Enseignement superieur
- Conseil Regional du Limousin
- Fondation pour la Recherche Medicale
- French National Research Agency [ANR-08-MIE-016]
- Institut National de la Sante et de la Recherche Medicale (Inserm)
- Association pour l'Organisation des Reunions Interdisciplinaires de Chimiotherapie Anti-infectieuse
- Pasteur Institute
- Centre National de la Recherche Scientifique [CNRS-URA 2171]
- EU [LSHM-CT-2005-019023]
- NoE EuroPathoGenomics [LSHB-CT-2005-512061]
Class 1 integrons are widespread genetic elements that allow bacteria to capture and express gene cassettes that are usually promoterless. These integrons play a major role in the dissemination of antibiotic resistance among Gram-negative bacteria. They typically consist of a gene (intl) encoding an integrase ( that catalyzes the gene cassette movement by site-specific recombination), a recombination site (attl1), and a promoter (Pc) responsible for the expression of inserted gene cassettes. The Pc promoter can occasionally be combined with a second promoter designated P2, and several Pc variants with different strengths have been described, although their relative distribution is not known. The Pc promoter in class 1 integrons is located within the intl1 coding sequence. The Pc polymorphism affects the amino acid sequence of Intl1 and the effect of this feature on the integrase recombination activity has not previously been investigated. We therefore conducted an extensive in silico study of class 1 integron sequences in order to assess the distribution of Pc variants. We also measured these promoters' strength by means of transcriptional reporter gene fusion experiments and estimated the excision and integration activities of the different Intl1 variants. We found that there are currently 13 Pc variants, leading to 10 Intl1 variants, that have a highly uneven distribution. There are five main Pc-P2 combinations, corresponding to five promoter strengths, and three main integrases displaying similar integration activity but very different excision efficiency. Promoter strength correlates with integrase excision activity: the weaker the promoter, the stronger the integrase. The tight relationship between the aptitude of class 1 integrons to recombine cassettes and express gene cassettes may be a key to understanding the short-term evolution of integrons. Dissemination of integron-driven drug resistance is therefore more complex than previously thought.
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