期刊
PLOS BIOLOGY
卷 10, 期 12, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.1001438
关键词
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资金
- NIH [1RC4GM096355-01]
- BCM Intellectual and Developmental Disabilities Research Center [NIH/NICHHD P30-HD024064]
- Edward and Josephine Hudson Scholarship Fund
- DB Program training grant [T32 HD055200]
- Nakajima Foundation
- Taiwan Merit Scholarships (TMS) Program
- National Science Council [NSC-095-SAF-I-564-015-TMS]
- REACH IRACDA Fellowship [5K12GM084897]
- Houston Laboratory and Population Science Training Program in Gene-Environment Interaction from Burroughs Wellcome Fund (BWF Grant) [1008200]
- [R01GM067858]
Rhodopsins (Rhs) are light sensors, and Rh1 is the major Rh in the Drosophila photoreceptor rhabdomere membrane. Upon photoactivation, a fraction of Rh1 is internalized and degraded, but it remains unclear how the rhabdomeric Rh1 pool is replenished and what molecular players are involved. Here, we show that Crag, a DENN protein, is a guanine nucleotide exchange factor for Rab11 that is required for the homeostasis of Rh1 upon light exposure. The absence of Crag causes a light-induced accumulation of cytoplasmic Rh1, and loss of Crag or Rab11 leads to a similar photoreceptor degeneration in adult flies. Furthermore, the defects associated with loss of Crag can be partially rescued with a constitutive active form of Rab11. We propose that upon light stimulation, Crag is required for trafficking of Rh from the trans-Golgi network to rhabdomere membranes via a Rab11-dependent vesicular transport.
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