期刊
PLOS BIOLOGY
卷 10, 期 9, 页码 -出版社
PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.1001396
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资金
- Medical Research Council United Kingdom [G0600089]
- Brain Research Trust
- Wellcome Trust [WT084311]
- European Research Council
- UCL Bridging Award
- Action Medical Research [1725] Funding Source: researchfish
- Medical Research Council [G0802158, G0501424, G0900613, G0802216, G116/147, G0600089, G0801316] Funding Source: researchfish
- MRC [G0802158, G0900613, G0501424, G116/147, G0802216, G0801316, G0600089] Funding Source: UKRI
The efficacy of action potential evoked neurotransmitter release varies widely even among synapses supplied by the same axon, and the number of release-ready vesicles at each synapse is a major determinant of this heterogeneity. Here we identify a second, equally important, mechanism for release heterogeneity at small hippocampal synapses, the inter-synaptic variation of the exocytosis probability of release-ready vesicles. Using concurrent measurements of vesicular pool sizes, vesicular exocytosis rates, and presynaptic Ca2+ dynamics, in the same small hippocampal boutons, we show that the average fusion probability of release-ready vesicles varies among synapses supplied by the same axon with the size of the spike-evoked Ca2+ concentration transient. We further show that synapses with a high vesicular release probability exhibit a lower Ca2+ cooperativity, arguing that this is a direct consequence of increased Ca2+ influx at the active zone. We conclude that variability of neurotransmitter release under basal conditions at small central synapses is accounted for not only by the number of release-ready vesicles, but also by their fusion probabilities, which are set independently of bouton size by variable spike-evoked presynaptic Ca2+ influx.
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