4.6 Article

Differentiation Driven Changes in the Dynamic Organization of Basal Transcription Initiation

期刊

PLOS BIOLOGY
卷 7, 期 10, 页码 -

出版社

PUBLIC LIBRARY SCIENCE
DOI: 10.1371/journal.pbio.1000220

关键词

-

资金

  1. European Molecular Biology Organization
  2. Erasmus University
  3. ZonMW [917-46-364, 912-03-012, 901-01-229]
  4. Association for International Cancer Research [07-0129]
  5. European Union [LSHG-CT-2005-512113, MRTN-CT-2003-503618]
  6. Midi Pyrenees [08017067]

向作者/读者索取更多资源

Studies based on cell-free systems and on in vitro-cultured living cells support the concept that many cellular processes, such as transcription initiation, are highly dynamic: individual proteins stochastically bind to their substrates and disassemble after reaction completion. This dynamic nature allows quick adaptation of transcription to changing conditions. However, it is unknown to what extent this dynamic transcription organization holds for postmitotic cells embedded in mammalian tissue. To allow analysis of transcription initiation dynamics directly into living mammalian tissues, we created a knock-in mouse model expressing fluorescently tagged TFIIH. Surprisingly and in contrast to what has been observed in cultured and proliferating cells, postmitotic murine cells embedded in their tissue exhibit a strong and long-lasting transcription-dependent immobilization of TFIIH. This immobilization is both differentiation driven and development dependent. Furthermore, although very statically bound, TFIIH can be remobilized to respond to new transcriptional needs. This divergent spatiotemporal transcriptional organization in different cells of the soma revisits the generally accepted highly dynamic concept of the kinetic framework of transcription and shows how basic processes, such as transcription, can be organized in a fundamentally different fashion in intact organisms as previously deduced from in vitro studies.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据