期刊
OPEN BIOLOGY
卷 3, 期 -, 页码 -出版社
ROYAL SOC
DOI: 10.1098/rsob.130002
关键词
Francisella tularensis; vaccine; glycoconjugate; protein glycan coupling technology
资金
- Defence Science and Technology Laboratories
- Biotechnology and Biological Research Council, United Kingdom
- Biotechnology and Biological Sciences Research Council [BB/H017437/1] Funding Source: researchfish
- BBSRC [BB/H017437/1] Funding Source: UKRI
Glycoconjugate-based vaccines have proved to be effective at producing long-lasting protection against numerous pathogens. Here, we describe the application of bacterial protein glycan coupling technology (PGCT) to generate a novel recombinant glycoconjugate vaccine. We demonstrate the conjugation of the Francisella tularensis O-antigen to the Pseudomonas aeruginosa carrier protein exotoxin A using the Campylobacter jejuni PglB oligosaccharyltransferase. The resultant recombinant F. tularensis glycoconjugate vaccine is expressed in Escherichia coli where yields of 3 mg l(-1) of culture were routinely produced in a single-step purification process. Vaccination of BALB/c mice with the purified glycoconjugate boosted IgG levels and significantly increased the time to death upon subsequent challenge with F. tularensis subsp. holarctica. PGCT allows different polysaccharide and protein combinations to be produced recombinantly and could be easily applicable for the production of diverse glycoconjugate vaccines.
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