期刊
JOURNAL OF PHARMACOLOGICAL AND TOXICOLOGICAL METHODS
卷 69, 期 3, 页码 211-216出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.vascn.2014.02.001
关键词
Ractopamine; Hair; Liquid chromatography; Validation
资金
- National Natural Science Foundation of China [31000762, 31271940]
A quantitative analytical procedure for the determination of ractopamine in pig hair has been developed and validated. The hair samples were washed and incubated at 75 degrees C with isoxuprine and hair extraction buffer. The drug present was quantified using mixed solid-phase extraction and liquid chromatography with tandem mass spectrometric detection. The limit of quantization (LOQ) was 10 pg/mg and the intra-day precision at 25 pg/mg and 750 pg/mg was 0.49% and 2.8% respectively. Inter-day precision was 0.88% and 3.52% at the same concentrations. The hair extraction percentage recovery at 25 pg/mg and 50 ng/mL was 99.47% and 103.83% respectively. The extraction percentage recovery at 25 pg/mg and 50 ng/mg was 93.52% and 100.26% respectively. Our results showed that ractopamine residues persist in hair in 24 days of withdrawal and also showed the possibility to test ractopamine from pig hair samples. (C) 2014 Elsevier Inc. All rights reserved.
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