期刊
JOURNAL OF INNOVATIVE OPTICAL HEALTH SCIENCES
卷 1, 期 2, 页码 207-215出版社
WORLD SCIENTIFIC PUBL CO PTE LTD
DOI: 10.1142/S1793545808000212
关键词
Photoacoustic; molecular imaging; gene expression; reporter gene
资金
- NIH [R01 NS46214, R01 EB000712]
Photoacoustic molecular imaging, combined with the reporter-gene technique, can provide a valuable tool for cancer research. The expression of the lacZ reporter gene can be imaged using photoacoustic imaging following the injection of X-gal, a colorimetric assay for the lacZ-encoded enzyme beta-galactosidase. Dual-wavelength photoacoustic microscopy was used to non-invasively image the detailed morphology of a lacZ-marked 9L gliosarcoma and its surrounding microvasculature simultaneously in vivo, with a superior resolution on the order of 10 mu m. Tumor-feeding vessels were found, and the expression level of lacZ in tumor was estimated. With future development of new absorption-enhancing reporter-gene systems, we anticipate this strategy can lead to a better understanding of the role of tumor metabolism in cancer initiation, progression, and metastasis, and in its response to therapy.
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