期刊
JOURNAL OF GINSENG RESEARCH
卷 38, 期 1, 页码 47-51出版社
KOREAN SOC GINSENG
DOI: 10.1016/j.jgr.2013.11.008
关键词
Aspergillus niger; beta-glucosidase; ginsenoside Rg3; Panax ginseng; transformation
资金
- Technology Development Program through the Ministry for Food, Agriculture, Forestry and Fisheries [610002-03-02-SB220]
- Priority Research Centers Program through the National Research Foundation of Korea (NRF) [2009-0093824]
- Ministry of Education, Science and Technology, Republic of Korea
The transformation of ginsenoside Rb1 into a specific minor ginsenoside using Aspergillus niger KCCM 11239, as well as the identification of the transformed products and the pathway via thin layer chromatography and high performance liquid chromatography were evaluated to develop a new biologically active material. The conversion of ginsenoside Rb1 generated Rd, Rg3, Rh2, and compound K although the reaction rates were low due to the low concentration. In enzymatic conversion, all of the ginsenoside Rb1 was converted to ginsenoside Rd and ginsenoside Rg3 after 24 h of incubation. The crude enzyme (beta-glucosidase) from A. niger KCCM 11239 hydrolyzed the beta-(1 -> 6)-glucosidic linkage at the C-20 of ginsenoside Rb1 to generate ginsenoside Rd and ginsenoside Rg3. Our experimental demonstration showing that A. niger KCCM 11239 produces the ginsenoside-hydrolyzing beta-glucosidase reflects the feasibility of developing a specific bioconversion process to obtain active minor ginsenosides. Copyright (C) 2013, The Korean Society of Ginseng, Published by Elsevier. All rights reserved.
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