期刊
JOURNAL OF CENTRAL SOUTH UNIVERSITY
卷 20, 期 4, 页码 1015-1021出版社
JOURNAL OF CENTRAL SOUTH UNIV
DOI: 10.1007/s11771-013-1578-8
关键词
Pseudomonas aeruginosa; rhamnolipid; waste frying oil; response surface method; fed-batch
资金
- Ministry of Education of China [108100]
- National Natural Science Foundation of China [50978087, 50908081]
- Hunan University Graduate Education Innovation Program, China [531107011019]
- Hunan Provincial Innovation Foundation for Postgraduate students, China [CX2010B157]
Rhamnolipid production by Pseudomonas aeruginosa ATCC 9027 with waste frying oil as sole carbon source was studied using response surface method. Cultures were incubated in shaking flask with temperature, NO3- and Mg2+ concentrations as the variables. Meanwhile, fed-batch fermentation experiments were conducted. The results show that the three variables are closely related to rhamnolipid production. The optimal cultivation conditions are of 6.4 g/L NaNO3, 3.1 g/L MgSO4 at 32 degrees C, with the maximum rhamnolipid production of 6.6 g/L. The results of fed-batch fermentation experiments show that feeding the oil in two batches can enhance rhamnolipid production. The best time interval is 72 h with the maximum rhamnolipid production of 8.5 g/L. The data are potentially useful for mass production of rhamnolipid on oil waste with this bacterium.
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