期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 104, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/53364
关键词
Infection; Issue 104; Arabidopsis thaliana; P. syringae pv. maculicola ES4326; infection; syringe infiltration; plant immunity; salicylic acid (SA); Enhanced Disease Susceptibility; Systemic Acquired Resistance; Microbe-Associated Molecular Patterns (MAMPs); MAMP-Triggered Immunity; SA-Triggered Immunity
资金
- NSF-CAREER award [IOS-1350244]
- UAB Biology Department
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [1350244] Funding Source: National Science Foundation
In the absence of specialized mobile immune cells, plants utilize their localized programmed cell death and Systemic Acquired Resistance to defend themselves against pathogen attack. The contribution of a specific Arabidopsis gene to the overall plant immune response can be specifically and quantitatively assessed by assaying the pathogen growth within the infected tissue. For over three decades, the hemibiotrophic bacterium Pseudomonas syringae pv. maculicola ES4326 (Psm ES4326) has been widely applied as the model pathogen to investigate the molecular mechanisms underlying the Arabidopsis immune response. To deliver pathogens into the leaf tissue, multiple inoculation methods have been established, e.g., syringe infiltration, dip inoculation, spray, vacuum infiltration, and flood inoculation. The following protocol describes an optimized syringe infiltration method to deliver virulent Psm ES4326 into leaves of adult soil-grown Arabidopsis plants and accurately screen for enhanced disease susceptibility (EDS) towards this pathogen. In addition, this protocol can be supplemented with multiple pre-treatments to further dissect specific immune defects within different layers of plant defense, including Salicylic Acid (SA)-Triggered Immunity (STI) and MAMP-Triggered Immunity (MTI).
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