期刊
JOURNAL OF IMMUNOLOGY RESEARCH
卷 2015, 期 -, 页码 -出版社
HINDAWI LTD
DOI: 10.1155/2015/361604
关键词
-
类别
资金
- Agencia Nacional de Promocion Cientifica y Tecnologica [PICT 00479/06]
- CONICET
- Universidad Nacional de La Plata [11X/548]
- Bordeaux Science Agro
- Ministere de l'Agriculture Francais
- Boehringer Ingelheim Fonds
The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-gamma, GM-CSF, and IL-1 beta genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据