期刊
BEHAVIOURAL BRAIN RESEARCH
卷 286, 期 -, 页码 64-70出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbr.2015.02.040
关键词
Hippocampus; Sleep deprivation; Glutamate receptor; T-maze
资金
- National Natural Science Foundation of China [81301137]
Hippocampus-dependent learning memory is sensitive to sleep deprivation (SD). Although the ionotropic glutamate receptors play a vital role in synaptic plasticity and learning and memory, however, whether the expression of these receptor subunits is modulated by sleep loss remains unclear. In the present study, western blotting was performed by probing with specific antibodies against the ionotropic cxamino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor subunits GluA1, GluA2, GluA3, and against the N-methyl-o-aspartate (NMDA) glutamate receptor subunits GluN1, GluN2A, GluN2B. In hippocampus, down regulation of surface GluA1 and GluN2A surface expression were observed in both SD groups. However, surface expression level of GluA2, GluA3, GluN1 and GluN2B was significantly upregulated in 8 h-SD rats when compared to the 4 h-SD rats. In parallel with the complex changes in AMPA and NMDA receptor subunit expressions, we found the 8 h-SD impaired rat spatial working memory in 30-s-delay T-maze task, whereas no impairment of spatial learning was observed in 4 h-SD rats. These results indicate that sleep loss alters the relative expression levels of the AMPA and NMDA receptors, thus affects the synaptic strength and capacity for plasticity and partially contributes to spatial memory impairment. (C) 2015 Published by Elsevier B.V.
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