期刊
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
卷 9, 期 11, 页码 4017-4032出版社
MDPI
DOI: 10.3390/ijerph9114017
关键词
real-time RT-PCR; enteric RNA viruses; surface waters; detection; quantification; hepatitis A virus; rotaviruses; enteroviruses
资金
- Water Research Commission (WRC) of South Africa
- Education Trust Fund of Nigeria
Human enteric viruses (HEntVs) are a major cause of water-related diseases. The prevalence of hepatitis A virus (HAV), rotaviruses (RoV) and enteroviruses (EnV) in Buffalo River waters was assessed quantitatively over a period of 12 months (August 2010 to July 2011). Seventy-two samples were collected from six sites, including three dams, and concentrated using the adsorption-elution method. Viral RNA was extracted using a commercial kit, and the viruses were quantified by real-time quantitative reverse transcriptase PCR (RT-qPCR). Two or more viruses were detected in 12.5% of the samples. HAV was detected in 43.1% of the samples and in significantly (p < 0.05) varying concentrations of 1.5 x 10(1)-1.9 x 10(5) genome copies/L compared to RoV and EnV, while RoVs were detected in 13.9% of samples, with concentrations ranging from 2.5 x 10(1)-2.1 x 10(3) genome copies/L, and EnV were detected in 9.7% of the samples, with concentrations ranging from 1.3 x 10(1)-8.6 x 10(1) genome copies/L. Only HAV was detected at all the sites, with the Bridle Drift Dam recording significantly higher (p < 0.05) concentrations. The presence of enteric viruses in Buffalo River may constitute public health risks and the incidence of HAV at all the sites could reflect both the epidemiological status of hepatitis A and HAV persistence in the water environments.
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