Flowering of Adonis amurensis by breaking dormancy using gibberellins and cytokinins

Amur adonis (Adonis amurensis) was used for the study of PGRs responses on the dormancy breaking and flowering. All plant treated with GA(3) or GA(4+7) showed earlier sprouting by breaking the dormancy and resulted to fast flowering, irrespective of concentration. Plant height and the number of nodes tent to be increased by GAs application on dormant plant. Among the different kinds of GA treatments, GA(4+7) was most effective in dormancy breaking and flower emergence with the effect of higher plant height and increased node number rather than GA(3). However, GA(4+7) showed several disadvantages such as reducing the flowering rate and induced the overgrowth that resulting in stretched weak plants. Beside GA, cytokinins such as TDZ showed clear effect of dormancy breaking but, other cytokinins such as BAP and Kinetin showed no clear positive effects in breaking the dormancy. Dormant amur adonis treated with BAP and Kinetin flowered without leaf emergency and subsequent shoot growth resulted in showing abnormal flowering and growth, but it treated with TDZ showed the normal flowering with shoot growth, but flowering percentage was very low. In conclusion, we could recommend that 100-500 mg center dot L-1 GA(3) application as an alternative method to chilling treatment for breaking dormancy and normal flowering of A. amurensis. And we could also recommend 20 or 50 mg center dot L-1 TDZ for the dormancy break and subsequent shoot growth, but not for flowering.