期刊
G3-GENES GENOMES GENETICS
卷 3, 期 10, 页码 1827-1832出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.113.007682
关键词
transgene induction; heat shock; C.elegans
资金
- National Institutes of Health (NIH)
- Office of Research Infrastructure Programs [P40 OD010440]
- Penn Comprehensive Neuroscience Center pilot
- P. Sloan Research Fellowship
- NIH [OD010440]
- Penn Genome Frontiers Institute
- Pennsylvania Department of Health
The coupling of transgenes to heat shock promoters is a widely applied method for regulating gene expression. In C. elegans, gene induction can be controlled temporally through timing of heat shock and spatially via targeted rescue in heat shock mutants. Here, we present a method for evoking gene expression in arbitrary cells, with single-cell resolution. We use a focused pulsed infrared laser to locally induce a heat shock response in specific cells. Our method builds on and extends a previously reported method using a continuous-wave laser. In our technique, the pulsed laser illumination enables a much higher degree of spatial selectivity because of diffusion of heat between pulses. We apply our method to induce transient and long-term transgene expression in embryonic, larval, and adult cells. Our method allows highly selective spatiotemporal control of transgene expression and is a powerful tool for model organism biology.
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