期刊
G3-GENES GENOMES GENETICS
卷 3, 期 3, 页码 399-407出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.112.005355
关键词
mutation rate; evolution; replication fidelity; chromosome structure; DNA polymerase errors
资金
- Multidisciplinary University Research Initiative Award from the U.S. Army Research Office [W911NF-09-1-0444]
By sequencing the genomes of 34 mutation accumulation lines of a mismatch-repair defective strain of Escherichia coli that had undergone a total of 12,750 generations, we identified 1625 spontaneous base-pair substitutions spread across the E. coli genome. These mutations are not distributed at random but, instead, fall into a wave-like spatial pattern that is repeated almost exactly in mirror image in the two separately replicated halves of the bacterial chromosome. The pattern is correlated to genomic features, with mutation densities greatest in regions predicted to have high superhelicity. Superimposed upon this pattern are regional hotspots, some of which are located where replication forks may collide or be blocked. These results suggest that, as they traverse the chromosome, the two replication forks encounter parallel structural features that change the fidelity of DNA replication.
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