期刊
G3-GENES GENOMES GENETICS
卷 2, 期 1, 页码 79-82出版社
GENETICS SOCIETY AMERICA
DOI: 10.1534/g3.111.001321
关键词
reporter plasmids; Phi C31 integrase; fluorescent protein; Drosophila; Notch
资金
- BBSRC [BB/F00897X/1] Funding Source: UKRI
- MRC [G0800034, G0500926] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/F00897X/1] Funding Source: Medline
- Medical Research Council [G0500926, G0800034] Funding Source: Medline
Complex spatial and temporal regulation of gene activity is fundamental to development and homeostasis. The ability to decipher the DNA sequences that accurately coordinate gene expression is, therefore, of primary importance. One way to assess the functions of DNA elements entails their fusion to fluorescent reporter genes. This powerful approach makes it possible to visualize their regulatory capabilities when reintroduced into the developing animal. Transgenic studies in Drosophila have recently advanced with the introduction of site-specific, Phi C31 integrase-mediated approaches. However, most existing Drosophila reporter vectors are not compatible with this new approach and have become obsolete. Here we describe a new series of fluorescent reporter vectors optimized for use with Phi C31 transgenesis. By using these vectors to generate a set of Notch reporter fly lines, we demonstrate their efficacy in reporting the function of gene regulatory elements.
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