期刊
CROP BREEDING AND APPLIED BIOTECHNOLOGY
卷 11, 期 4, 页码 352-357出版社
BRAZILIAN SOC PLANT BREEDING
DOI: 10.1590/S1984-70332011000400009
关键词
genetic diversity; early molecular sexing
资金
- FINEP
- FAPERJ
- CNPq
ISSR markers are polymorphic and their results easily reproducible. They are therefore intensely used in phylogenetic studies and sex differentiation of some economically interesting plant species. The objectives of this study were to analyze the genetic diversity in Caricaceae using ISSR markers, to identify a specific ISSR band that could distinguish female from hermaphrodite papaya genotypes and to verify whether this marker could be used for early sex differentiation. The ISSR-PCR was performed with nine primers and they could distinguish all species. It was observed that Jacaratia spinosa was closer to Vasconcellea than to Carica. The species C. papaya was only distantly related to both genera. A 500 bp ISSR marker was found in 25 % of the papaya genotypes studied. Specifically in these cases this marker could be used for early sex differentiation in papaya.
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