期刊
CROP BREEDING AND APPLIED BIOTECHNOLOGY
卷 9, 期 4, 页码 361-371出版社
BRAZILIAN SOC PLANT BREEDING
DOI: 10.12702/1984-7033.v09n04a11
关键词
SSR marker; enriched genomic library; coffee; molecular marker
With the objective of developing new SSR markers for Coffea arabica, two enriched genomic libraries with probes (GT)(15) and (AAG)(10) were constructed A total of 835 clones were sequenced and 756 presented good quality sequences Redundant sequences were observed for 113 clones (14 94%) SSRs were found in 287 clones (38%) An estimated size of 417 5Kb of the C arabica genome was sampled, with an average of one SSR per 146Kb Dinucleotide repeats were more frequent than trinucleotides Four repeat sequences, (AG/CT)(n), (AC/GT)(n), (AAG/CTT)(n), and (AGG/CCT)(n) represented 61 1% of the total observed A total of 96 SSR primers were designed and tested by PCR for two CC arabica genotypes Ninety new SSR markers were validated for further genetic studies of C arabica
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