期刊
CELL REPORTS
卷 8, 期 1, 页码 311-318出版社
CELL PRESS
DOI: 10.1016/j.celrep.2014.05.056
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT) [25115729, 25117002, 24111551, 25116523]
- MEXT [25702054, 23680042]
- Takeda Science Foundation
- Grants-in-Aid for Scientific Research [25116523, 23680042, 26117501, 25115729, 24111551, 25117002, 25702054] Funding Source: KAKEN
Astrocytes generate local calcium (Ca2+) signals that are thought to regulate their functions. Visualization of these signals in the intact brain requires an imaging method with high spatiotemporal resolution. Here, we describe such a method using transgenic mice expressing the ultrasensitive ratiometric Ca2+ indicator yellow Cameleon-Nano 50 (YC-Nano50) in astrocytes. In these mice, we detected a unique pattern of Ca2+ signals. These occur spontaneously, predominantly in astrocytic fine processes, but not the cell body. Upon sensory stimulation, astrocytes initially responded with Ca2+ signals at fine processes, which then propagated to the cell body. These observations suggest that astrocytic fine processes function as a high-sensitivity detector of neuronal activities. Thus, the method provides a useful tool for studying the activity of astrocytes in brain physiology and pathology.
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