期刊
CELL REPORTS
卷 8, 期 5, 页码 1432-1446出版社
CELL PRESS
DOI: 10.1016/j.celrep.2014.07.035
关键词
-
类别
资金
- Fonds National Suisse de la Recherche Scientifique (SNSF grant) [31003A-143978]
- National Center of Competence in Research (NCCR) in Molecular Oncology
- European Research Council (ERC)
- Anna Fuller Fund
- Swiss Federal Government through the State Secretariat for Education, Research and Innovation (SERI)
- Swiss National Science Foundation (SNF) [31003A_143978] Funding Source: Swiss National Science Foundation (SNF)
MicroRNA (miRNA) transfer via exosomes may mediate cell-to-cell communication. Interestingly, specific miRNAs are enriched in exosomes in a cell-type-dependent fashion. However, the mechanisms whereby miRNAs are sorted to exosomes and the significance of miRNA transfer to acceptor cells are unclear. We used macrophages and endothelial cells (ECs) as a model of heterotypic cell communication in order to investigate both processes. RNA profiling of macrophages and their exosomes shows that miRNA sorting to exosomes is modulated by cell-activation-dependent changes of miRNA target levels in the producer cells. Genetically perturbing the expression of individual miRNAs or their targeted transcripts promotes bidirectional miRNA relocation from the cell cytoplasm/P bodies (sites of miRNA activity) to multivesicular bodies (sites of exosome biogenesis) and controls miRNA sorting to exosomes. Furthermore, the use of Dicer-deficient cells and reporter lentiviral vectors (LVs) for miRNA activity shows that exosomal miRNAs are transferred from macrophages to ECs to detectably repress targeted sequences.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据