期刊
CELL REPORTS
卷 4, 期 5, 页码 1035-1048出版社
CELL PRESS
DOI: 10.1016/j.celrep.2013.06.021
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资金
- Project A.L.S.
- P2ALS
- Swedish Brain Foundation/Hjarnfonden
- Portuguese Foundation for Science and Technology [SFRH/BD/33421/2008]
- Luso-American Development Foundation
- NINDS [R01 NS042269-05A2]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/33421/2008] Funding Source: FCT
Differentiation of astrocytes from human stem cells has significant potential for analysis of their role in normal brain function and disease, but existing protocols generate only immature astrocytes. Using early neuralization, we generated spinal cord astrocytes from mouse or human embryonic or induced pluripotent stem cells with high efficiency. Remarkably, short exposure to fibroblast growth factor 1 (FGF1) or FGF2 was sufficient to direct these astrocytes selectively toward a mature quiescent phenotype, as judged by both marker expression and functional analysis. In contrast, tumor necrosis factor alpha and interleukin-1 beta, but not FGFs, induced multiple elements of a reactive inflammatory phenotype but did not affect maturation. These phenotypically defined, scalable populations of spinal cord astrocytes will be important both for studying normal astrocyte function and for modeling human pathological processes in vitro.
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