期刊
CELL REPORTS
卷 4, 期 5, 页码 938-944出版社
CELL PRESS
DOI: 10.1016/j.celrep.2013.07.049
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资金
- Netherlands Organization for Scientific Research (NWO) via an ALW TOP grant [854.10.003]
- ALW Vidi grant [864.11.005]
- Ministere de l'Enseignement Superieur et de la Recherche de France
- U.S. Department of Health and Human Services intramural program (National Library of Medicine, NIH)
- Azrieli PhD fellowship
- ERC-StG grant [260432]
- Israeli Science Foundation [ISF-1303/12]
- Swedish Research Council
- Uppsala RNA Research Centre
- European Research Council (ERC) [260432] Funding Source: European Research Council (ERC)
Clustering of functionally related genes in operons allows for coregulated gene expression in prokaryotes. This is advantageous when equal amounts of gene products are required. Production of protein complexes with an uneven stoichiometry, however, requires tuning mechanisms to generate subunits in appropriate relative quantities. Using comparative genomic analysis, we show that differential translation is a key determinant of modulated expression of genes clustered in operons and that codon bias generally is the best in silico indicator of unequal protein production. Variable ribosome density profiles of polycistronic transcripts correlate strongly with differential translation patterns. In addition, we provide experimental evidence that de novo initiation of translation can occur at intercistronic sites, allowing for differential translation of any gene irrespective of its position on a polycistronic messenger. Thus, modulation of translation efficiency appears to be a universal mode of control in bacteria and archaea that allows for differential production of operon-encoded proteins.
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