期刊
CELL REPORTS
卷 2, 期 1, 页码 150-161出版社
CELL PRESS
DOI: 10.1016/j.celrep.2012.06.004
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类别
资金
- National Institutes of Health [DP1 OD000217, R01 GM085286, R01 GM085286-01S, GM81648, GM4336]
- La Fondation Pierre Gilles de Gennes
- National Science Foundation [DMR-0706458, MRSEC-0820492]
A canonical quantitative view of transcriptional regulation holds that the only role of operator sequence is to set the probability of transcription factor binding, with operator occupancy determining the level of gene expression. In this work, we test this idea by characterizing repression in vivo and the binding of RNA polymerase in vitro in experiments where operators of various sequences were placed either upstream or downstream from the promoter in Escherichia coli. Surprisingly, we find that operators with a weaker binding affinity can yield higher repression levels than stronger operators. Repressor bound to upstream operators modulates promoter escape, and the magnitude of this modulation is not correlated with the repressor-operator binding affinity. This suggests that operator sequences may modulate transcription by altering the nature of the interaction of the bound transcription factor with the transcriptional machinery, implying a new layer of sequence dependence that must be confronted in the quantitative understanding of gene expression.
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