期刊
CELL REPORTS
卷 1, 期 6, 页码 703-714出版社
CELL PRESS
DOI: 10.1016/j.celrep.2012.04.009
关键词
-
类别
资金
- European Community [22943]
- TransEuro [HEALTH-F5-2010-242003]
- Swedish Research Council
- Crafoord Foundation
- Swedish Parkinson Foundation
- Kocks Foundation
- Segerfalk Foundation
- Lundbeck Foundation [R44-A3856]
- [K2011-62X-20390-05-6]
- [349-2007-8626]
- Lundbeck Foundation [R44-2009-3856] Funding Source: researchfish
To model human neural-cell-fate specification and to provide cells for regenerative therapies, we have developed a method to generate human neural progenitors and neurons from human embryonic stem cells, which recapitulates human fetal brain development. Through the addition of a small molecule that activates canonical WNT signaling, we induced rapid and efficient dose-dependent specification of regionally defined neural progenitors ranging from telencephalic forebrain to posterior hindbrain fates. Ten days after initiation of differentiation, the progenitors could be transplanted to the adult rat striatum, where they formed neuron-rich and tumor-free grafts with maintained regional specification. Cells patterned toward a ventral midbrain (VM) identity generated a high proportion of authentic dopaminergic neurons after transplantation. The dopamine neurons showed morphology, projection pattern, and protein expression identical to that of human fetal VM cells grafted in parallel. VM-patterned but not forebrain-patterned neurons released dopamine and reversed motor deficits in an animal model of Parkinson's disease.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据