期刊
BENEFICIAL MICROBES
卷 4, 期 4, 页码 357-365出版社
WAGENINGEN ACADEMIC PUBLISHERS
DOI: 10.3920/BM2013.0026
关键词
inflammation; small intestine; NF kappa B; IL-1 beta; myeloperoxidase
资金
- University (Alberta) Hospital Foundation
- American College of Surgeons Faculty Research Fellowship
Acute intestinal ischaemia/reperfusion injury (AII/R) is an adaptive physiologic response during critical illness, involving mesenteric vasoconstriction and hypoperfusion. Prevention of AII/R in high risk patient populations would have a significant impact on morbidity and mortality. The purpose of this study was to investigate the protective effects of VSL#3 probiotic treatment in a murine model of AII/R. Adult 129/SvEv mice were subjected to an experimental AII/R model using superior mesenteric artery occlusion. Animals were pre-treated with either three days or two weeks of VSL#3 probiotics. Local tissue injury markers were assessed by levels of myeloperoxidase and activation of nuclear factor kappa B (NF kappa B). Systemic and local cytokines, including interleukin (IL)-1 beta, IL-10, TNF alpha, and interferon gamma were measured by ELISA and multiplex fluorescent detection. VSL#3 probiotics reduced local tissue inflammation and injury due to AII/R. A two-week course of VSL#3 was more effective than a shorter three-day course. The reduction in local inflammation from the two-week course of VSL#3 is correlated to a significant reduction in levels of active IL-1 beta, and tissue levels of myeloperoxidase. Levels of active NF kappa B were significantly elevated in the vehicle-fed AII/R mice, corroborating with tissue inflammation, which were attenuated by VSL#3 administrations. VSL#3 did not cause any systemic inflammation or lung injury. VSL#3 probiotics are effective in reducing local tissue injury from AII/R by down-regulating pro-inflammatory mediators and immune cell recruitment. This study highlights a potential role for VSL#3 in management of patients at high risk for AII/R.
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