期刊
ADVANCED HEALTHCARE MATERIALS
卷 3, 期 7, 页码 1036-1043出版社
WILEY
DOI: 10.1002/adhm.201300575
关键词
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资金
- National Institutes of Health [1 UH2 TR000522-01]
- Wyss Institute for Biologically Inspired Engineering
- Vertex Pharmaceuticals Incorporated
- MRSEC
In vitro models of ischemia have not historically recapitulated the cellular interactions and gradients of molecules that occur in a 3D tissue. This work demonstrates a paper-based 3D culture system that mimics some of the interactions that occur among populations of cells in the heart during ischemia. Multiple layers of paper containing cells, suspended in hydrogels, are stacked to form a layered 3D model of a tissue. Mass transport of oxygen and glucose into this 3D system can be modulated to induce an ischemic environment in the bottom layers of the stack. This ischemic stress induces cardiomyocytes at the bottom of the stack to secrete chemokines which subsequently trigger fibroblasts residing in adjacent layers to migrate toward the ischemic region. This work demonstrates the usefulness of patterned, stacked paper for performing in vitro mechanistic studies of cellular motility and viability within a model of the laminar ventricle tissue of the heart.
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