期刊
ADVANCED HEALTHCARE MATERIALS
卷 2, 期 8, 页码 1142-1150出版社
WILEY
DOI: 10.1002/adhm.201200458
关键词
self-assembly; photoencapsulation; micropatterning; tissue engineering; regenerative medicine
资金
- NSF [CBET-1066898]
- NIH Director's New Innovator Award Program [DP2-OD004346-01, DP2-OD004346-01S1]
- Ford Foundation Fellowship Program
- Iacocca Family Foundation
- Directorate For Engineering
- Div Of Chem, Bioeng, Env, & Transp Sys [1066898] Funding Source: National Science Foundation
We describe the self-folding of photopatterned poly (ethylene glycol) (PEG)-based hydrogel bilayers into curved and anatomically relevant micrometer-scale geometries. The PEG bilayers consist of two different molecular weights (MWs) and are photocrosslinked en masse using conventional photolithography. Self-folding is driven by differential swelling of the two PEG bilayers in aqueous solutions. We characterize the self-folding of PEG bilayers of varying composition and develop a finite element model which predicts radii of curvature that are in good agreement with empirical results. Since we envision the utility of bio-origami in tissue engineering, we photoencapsulate insulin secreting -TC-6 cells within PEG bilayers and subsequently self-fold them into cylindrical hydrogels of different radii. Calcein AM staining and ELISA measurements are used to monitor cell proliferation and insulin production respectively, and the results indicate cell viability and robust insulin production for over eight weeks in culture.
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