期刊
ADVANCED HEALTHCARE MATERIALS
卷 2, 期 10, 页码 1370-1376出版社
WILEY
DOI: 10.1002/adhm.201200370
关键词
dimers; gold nanoparticles; imaging; SERS; targeting
资金
- NSF NIRT BES [0609000]
- NIH EB [001046]
- Rutgers University
- Rutgers Faculty Research Grant Award [281656]
- Div Of Chem, Bioeng, Env, & Transp Sys
- Directorate For Engineering [0609000] Funding Source: National Science Foundation
Herein, a new class of multifunctional materials combining a clustered nanoparticle-based probe is presented for surface enhanced Raman scattering (SERS)-based microscopy and surface functionalization for tissue targeting. Controlled assembly of spherical gold nanoparticles into dimers (DNP-REP) is engineered using a small, rigid Raman-active dithiolated linking reporter (REP) to yield narrow internanoparticle gaps and to strategically generate the hot spot while concurrently placing the reporter within the region of highest SERS enhancement. Peptide functionalized DNP-REP materials are highly stable even upon incubation with living cells and show controlled levels of binding and intracellular endocytosis. To demonstrate the functionality of such probes for disease detection, differentially targeted DNP-REPs are incubated over various time points with cultured human glioblastoma cells. Using human glioblastoma cells, the SERS maps of targeted tumor cells show the markedly enhanced signals of the DNP-REP, compared to conventional confocal fluorescence based approaches, especially at low incubation times. Even with as few as 40 internalized DNP-REP, a relatively intense SERS signal is measured, demonstrating the high signal to noise ratio and inherent biocompatibility of the materials. Thus, these Raman reporter-based nanoparticle cluster probes present a promising and versatile optical imaging tool for fast, reliable, selective, and ultrasensitive tissue targeting and disease detection and screening.
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