期刊
ADVANCED HEALTHCARE MATERIALS
卷 2, 期 11, 页码 1489-1500出版社
WILEY
DOI: 10.1002/adhm.201200489
关键词
biodegradation; confocal Raman imaging; graphene; k-means cluster analysis; macrophages
资金
- Department of Science and Technology (DST), Govt. of India
- Department of Biotechnology, Govt. of India
- Nanotoxicolgy and Nanomedicine, Phase-II [BT/PR14920/NNT/28/503/2010]
- Council of Scientific and Industrial Research, Govt. of India [9/963(0015)2K11-EMR1]
This study is focused on the crucial issue of biodegradability of graphene under in vivo conditions. Characteristic Raman signatures of graphene are used to three dimensionally (3D) image its localization in lung, liver, kidney and spleen of mouse and identified gradual development of structural disorder, happening over a period of 3 months, as indicated by the formation of defect-related D'band, line broadening of D and G bands, increase in I-D/I-G ratio and overall intensity reduction. Prior to injection, the carboxyl functionalized graphene of lateral size approximate to 200 nm is well dispersed in aqueous medium, but 24 hours post injection, larger aggregates of size up to 10 m are detected in various organs. Using Raman cluster imaging method, temporal development of disorder is detected from day 8 onwards, which begins from the edges and grows inwards over a period of 3 months. The biodegradation is found prominent in graphene phagocytosed by tissue-bound macrophages and the gene expression studies of pro-inflammatory cytokines indicated the possibility of phagocytic immune response. In addition, in vitro studies conducted on macrophage cell lines also show development of structural disorder in the engulfed graphene, reiterating the role of macrophages in biodegradation. This is the first report providing clear evidence of in vivo biodegradation of graphene and these results may radically change the perspective on potential biomedical applications of graphene.
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