期刊
ACS SYNTHETIC BIOLOGY
卷 7, 期 9, 页码 2074-2086出版社
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00251
关键词
algal biotechnology; Chlamydomonas reinhardtii; modular cloning; synthetic biology
资金
- Agence Nationale de la Recherche [ANR-17-CE05-0008]
- LABEX DYNAMO [ANR-LABX-011]
- DFG [TRR175, FOR2092]
- OpenPlant (BBSRC/EPSRC)
- Ministerio de Economia y Competitividad [BFU2015-68216-P, BIO2015-74432-JIN]
- VILLUM Foundation [13363]
- Technology Platforms at the Center for Biotechnology (CeBiTec) Bielefeld University
- UK Biotechnology and Biological Sciences Research Council (BBSRC)
- ERA-SynBio project Sun2Chem
- BBSRC [BB/M018180/1, BB/I007660/1, BB/I00680X/1, BB/L014130/1, BB/R021694/1, BB/M018210/1, BB/D011043/1] Funding Source: UKRI
Microalgae are regarded as promising organisms to develop innovative concepts based on their photosynthetic capacity that offers more sustainable production than heterotrophic hosts. However, to realize their potential as green cell factories, a major challenge is to make microalgae easier to engineer. A promising approach for rapid and predictable genetic manipulation is to use standardized synthetic biology tools and workflows. To this end we have developed a Modular Cloning toolkit for the green microalga Chlamydomonas reinhardtii. It is based on Golden Gate cloning with standard syntax, and comprises 119 openly distributed genetic parts, most of which have been functionally validated in several strains. It contains promoters, UTRs, terminators, tags, reporters, antibiotic resistance genes, and introns cloned in various positions to allow maximum modularity. The toolkit enables rapid building of engineered cells for both fundamental research and algal biotechnology. This work will make Chlamydomonas the next chassis for sustainable synthetic biology.
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