期刊
ACS SYNTHETIC BIOLOGY
卷 4, 期 2, 页码 150-161出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb500024b
关键词
feedback circuits; negative feedback; synthetic protein scaffolds; two-component systems; live-cell tracking
资金
- DoD, Air Force Office of Scientific Research, National Defense Science and Engineering Graduate (NDSEG) Fellowship [32 CFR 168a]
- Benjamin M. Rosen Bioengineering Center
- NIH/NRSA Training Grant [S T32 GM07616]
- Gordon and Betty Moore Foundation [GBMF2809]
- Institute for Collaborative Biotechnologies from the U.S. Army Research Office [W911NF-09-0001]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1317694] Funding Source: National Science Foundation
As a field, synthetic biology strives to engineer increasingly complex artificial systems in living cells. Active feedback in closed loop systems offers a dynamic and adaptive way to ensure constant relative activity independent of intrinsic and extrinsic noise. In this work, we use synthetic protein scaffolds as a modular and tunable mechanism for concentration tracking through negative feedback. Input to the circuit initiates scaffold production, leading to colocalization of a two-component system and resulting in the production of an inhibitory antiscaffold protein. Using a combination of modeling and experimental work, we show that the biomolecular concentration tracker circuit achieves dynamic protein concentration tracking in Escherichia coli and that steady state outputs can be tuned.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据