期刊
ACS SYNTHETIC BIOLOGY
卷 4, 期 10, 页码 1070-1076出版社
AMER CHEMICAL SOC
DOI: 10.1021/sb500299c
关键词
directed evolution; transcription; genetic circuits
资金
- Welch Foundation [F-1654]
- National Security Science and Engineering Faculty Fellowship [FA9550-10-1-0169]
T7 RNA polymerase is the foundation of synthetic biological circuitry both in vivo and in vitro due to its robust and specific control of transcription from its cognate promoter. Here we present the directed evolution of a panel of orthogonal T7 RNA polymerase:promoter pairs that each specifically recognizes a synthetic promoter. These newly described pairs can be used to independently control up to six circuits in parallel.
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